FIGURE

Fig. 10

ID

ZDB-FIG-110531-24

Publication

Yeh et al., 2011 - Ptenb Mediates Gastrulation Cell Movements via Cdc42/AKT1 in Zebrafish

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Fig. 10

Knockdown of ptenb enhances actin polymerization.

The 10 ng StdMO (A) and 10 ng ptenb tMO₁ (B)-treated embryos were fixed at 70% epiboly, stained with rhodamine-phalloidin to reveal the F-actin, observed under confocal microscopy, and photographed with vegetal view. The F-actin contents of shield stage-embryos (n = 60) were measured by actin polymerization assay as described in “Materials and Methods”. Relative fluorescence (Rf) values from three independent experiments were corrected by subtracting background Rf. * indicates the value is significantly different from that of the StdMO treatment (p<0.05). Scale bar: 25 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TU + MO1-ptenb
Knockdown Reagent:	MO1-ptenb
Observed In:	actin filament polymerization
Stage:	Shield

Phenotype Detail

Acknowledgments

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