ZFIN ID: ZDB-FIG-111028-32
Dalgin et al., 2011 - Zebrafish mnx1 controls cell fate choice in the developing endocrine pancreas. Development (Cambridge, England)   138(21):4597-4608 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 8 Beta cell progenitors take on an alpha cell fate in Mnx1-deficient embryos. (A-P) Confocal images of 72 hpf Tg(mnx1:GFP) zebrafish embryos injected with control MO (A-D,I-L) or Mnx1 MO2 (E-H,M-P). (A-H) Whole-mount immunolabeling for GFP (green) and insulin (red), with nuclear staining with TO-PRO-3 (blue). (I-P) Whole-mount immunostaining for GFP (green) and glucagon (red), with nuclear staining with TO-PRO-3 (blue). Arrowheads indicate colocalization of glucagon and GFP. Data are representative of three independent experiments with a minimum of 20 embryos per group.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP os26Tg control Protruding-mouth Not Detected endocrine pancreas pancreatic A cell IHC
Protruding-mouth endocrine pancreas pancreatic B cell IHC
os26Tg + MO2-mnx1 standard conditions Protruding-mouth endocrine pancreas pancreatic A cell IHC
Protruding-mouth endocrine pancreas pancreatic B cell IHC
ins Ab1-ins os26Tg control Protruding-mouth endocrine pancreas pancreatic B cell IHC
Ab1-ins os26Tg + MO2-mnx1 standard conditions Protruding-mouth endocrine pancreas pancreatic B cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-gcg IHC os26Tg control Protruding-mouth endocrine pancreas pancreatic A cell
IHC os26Tg + MO2-mnx1 standard conditions Protruding-mouth endocrine pancreas pancreatic A cell
Phenotype Details
Fish Conditions Stage Phenotype
os26Tg + MO2-mnx1 standard conditions Protruding-mouth endocrine pancreas has extra parts of type pancreatic A cell, abnormal
Protruding-mouth endocrine pancreas has fewer parts of type pancreatic B cell, abnormal
Protruding-mouth pancreatic A cell differentiation increased occurrence, abnormal
Protruding-mouth type B pancreatic cell differentiation decreased occurrence, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development