ZFIN ID: ZDB-FIG-111028-32 |
Dalgin et al., 2011
- Zebrafish mnx1 controls cell fate choice in the developing endocrine pancreas.
Development (Cambridge, England)
138(21):4597-4608
Full text @ Development
EXPRESSION / LABELING:
Genes: | |
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Antibodies: | |
Fish: | |
Knockdown Reagent: | |
Anatomical Terms: | |
Stage: | Protruding-mouth |
PHENOTYPE:
Fish: | |
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Knockdown Reagent: | |
Observed In: | |
Stage: | Protruding-mouth |
Fig. 8 Beta cell progenitors take on an alpha cell fate in Mnx1-deficient embryos. (A-P) Confocal images of 72 hpf Tg(mnx1:GFP) zebrafish embryos injected with control MO (A-D,I-L) or Mnx1 MO2 (E-H,M-P). (A-H) Whole-mount immunolabeling for GFP (green) and insulin (red), with nuclear staining with TO-PRO-3 (blue). (I-P) Whole-mount immunostaining for GFP (green) and glucagon (red), with nuclear staining with TO-PRO-3 (blue). Arrowheads indicate colocalization of glucagon and GFP. Data are representative of three independent experiments with a minimum of 20 embryos per group. |
Gene Expression Details
Gene | Antibody | Fish | Conditions | Stage | Qualifier | Anatomy | Assay |
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EGFP | os26Tg | control | Protruding-mouth | Not Detected | endocrine pancreas pancreatic A cell | IHC | |
Protruding-mouth | endocrine pancreas pancreatic B cell | IHC | |||||
os26Tg + MO2-mnx1 | standard conditions | Protruding-mouth | endocrine pancreas pancreatic A cell | IHC | |||
Protruding-mouth | endocrine pancreas pancreatic B cell | IHC | |||||
ins | Ab1-ins | os26Tg | control | Protruding-mouth | endocrine pancreas pancreatic B cell | IHC | |
Ab1-ins | os26Tg + MO2-mnx1 | standard conditions | Protruding-mouth | endocrine pancreas pancreatic B cell | IHC |
Antibody Labeling Details
Antibody | Assay | Fish | Conditions | Stage | Anatomy |
---|---|---|---|---|---|
Ab1-gcg | IHC | os26Tg | control | Protruding-mouth | endocrine pancreas pancreatic A cell |
IHC | os26Tg + MO2-mnx1 | standard conditions | Protruding-mouth | endocrine pancreas pancreatic A cell |
Phenotype Details
Acknowledgments: | This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development |
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