Fig. 8 Beta cell progenitors take on an alpha cell fate in Mnx1-deficient embryos. (A-P) Confocal images of 72 hpf Tg(mnx1:GFP) zebrafish embryos injected with control MO (A-D,I-L) or Mnx1 MO2 (E-H,M-P). (A-H) Whole-mount immunolabeling for GFP (green) and insulin (red), with nuclear staining with TO-PRO-3 (blue). (I-P) Whole-mount immunostaining for GFP (green) and glucagon (red), with nuclear staining with TO-PRO-3 (blue). Arrowheads indicate colocalization of glucagon and GFP. Data are representative of three independent experiments with a minimum of 20 embryos per group.