FIGURE

Fig. 8

ID
ZDB-FIG-111028-19
Publication
Palencia-Desai et al., 2011 - Vascular endothelial and endocardial progenitors differentiate as cardiomyocytes in the absence of Etsrp/Etv2 function
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Fig. 8

Simultaneous Foxc1a and Etsrp knockdown affects endocardial but not myocardial development. (A-L) Endocardial markers kdrl:GFP at 30 hpf (A-D, arrows), kdrl at the 20-somite stage (E-H, arrows) and nfatc1 at 30 hpf (I-L, arrows) show no or only a slight reduction in expression in embryos injected with low doses of either 2 ng Etsrp MO (B,F,J) or 2 ng Foxc1a MO (C,G,K) compared with uninjected controls (A,E,I). However, upon simultaneous knockdown of Etsrp and Foxc1a, levels of all of these endocardial markers are severely reduced (D,H,L). (M-T) Myocardial hand2 expression at the 10-somite stage (M-P, arrows show anterior border) and cmlc2 at the 20-somite stage (Q-T) are not significantly affected in 2 ng etsrp MO-injected (N,R), 2 ng Foxc1a MO-injected (O,S) or Etsrp/Foxc1a MO co-injected (P,T) embryos compared with uninjected controls (M,Q). (A-D) Whole-mount fluorescent images of fixed Tg(kdrl:GFP) embryos at 30 hpf, anterior is towards the left, dorsal is upwards. (E-P) Ventral flat-mount view of in situ hybridization analysis, anterior is upwards. (Q-T) Anterodorsal whole-mount view, anterior is upwards. (U) Phenotypic distribution of endocardial kdrl:GFP, kdrl, nfatc1 and myocardial hand2 expression in etsrp, foxc1a and etsrp/foxc1a MO-injected embryos. The blue bars represent no change, whereas the green and red bars indicate mild and severe reduction in endocardial expression, respectively. Note that for kdrl:GFP and kdrl expression, normal or mildly reduced categories are combined and shown in dark green because it was difficult to distinguish the two categories.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 20-25 somites to Prim-15

Phenotype Detail
Acknowledgments
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