FIGURE
Fig. 2
- ID
- ZDB-FIG-110720-31
- Publication
- Hsu et al., 2011 - Zebrafish Cyclin-Dependent Protein Kinase-Like 1 (zcdkl1): Identification and Functional Characterization
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Fig. 2
Kinase activity assay of zcdkl1 in HEK 293 cells transfected with pEGFP or pEGFP-zcdkl1. Twenty-four h post-transfection, cells were lysed with lysis buffer and subjected to immunoprecipitation with anti-GFP antibody. (A) Immunoprecipitated complexes underwent in vitro kinase assay using myelin basic protein (MBP) and histone H1 as substrates. Phosphorylation of histone H1 (lane 1 and 3) and MPB (lanes 2 and 4) was detected by autoradiography; (B) Immunoprecipitated complexes (lane 1 for GFP alone; lane 2 for GFP-zcdkl1) were subjected to Western blot analysis using anti-GFP antibodies. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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Full text @ Int. J. Mol. Sci.