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Fig. 2
Kinase activity assay of zcdkl1 in HEK 293 cells transfected with pEGFP or pEGFP-zcdkl1. Twenty-four h post-transfection, cells were lysed with lysis buffer and subjected to immunoprecipitation with anti-GFP antibody. (A) Immunoprecipitated complexes underwent in vitro kinase assay using myelin basic protein (MBP) and histone H1 as substrates. Phosphorylation of histone H1 (lane 1 and 3) and MPB (lanes 2 and 4) was detected by autoradiography; (B) Immunoprecipitated complexes (lane 1 for GFP alone; lane 2 for GFP-zcdkl1) were subjected to Western blot analysis using anti-GFP antibodies.
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