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Fig. S1

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ZDB-FIG-110624-5

Publication

Row et al., 2011 - Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail

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Fig. S1

Mesoderm marker gene expression in induced ventral/lateral mesoderm embryos. The utility of the induced ventral/lateral mesoderm system was demonstrated by analyzing marker gene expression in treated embryos. Mesodermal identity was assayed by in situ hybridization for transcripts typical of ventral/lateral mesoderm fate (no tail (ntl), spadetail (spt) and paraxial protocadherin (papc)) or axial mesoderm fate (floating head (flh)). Treated embryos greatly expand their expression of ventral/lateral marker genes while axial fates are repressed. Blocking expression of spt with morpholino injection expands the domain of ntl expression and partially blocks papc expression, similar to what is observed in the mesoderm of spt embryos. The view is lateral with the dorsal pole to the right when it could be identified.

Expression Data

Expression Detail

Antibody Labeling

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Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 354(1), Row, R.H., Maître, J.L., Martin, B.L., Stockinger, P., Heisenberg, C.P., and Kimelman, D., Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail, 102-110, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.