FIGURE

Fig. 5

ID

ZDB-FIG-110622-16

Publication

Alibaud et al., 2011 - A Mycobacterium marinum TesA mutant defective for major cell wall associated lipids is highly attenuated in Dictyostelium discoideum and zebrafish embryos

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Fig. 5

Infection of zebrafish with the Mma tesA::Tn mutant: CHT injections. A. Fish survival was scored for each of three sets of injected embryos (n = 24). P < 0.0001 for MmaM versus tesA::Tn and MmaM versus RD1. These results are representative of three independent experiments. B. Whole embryo bacterial counts of MmaM, RD1 or tesA::Tn-infected embryos. Results are expressed as mean cfu per fish ± SEM from three independent experiments. Inoculates were MmaM (91 ± 40), RD1 (172 ± 130), tesA::Tn (144 ± 16). At 7 dpi, P < 0.01 for MmaM versus tesA::Tn and P < 0.05 for MmaM versus RD1. The symbols used are the same as those presented in (A). C. Overlay of fluorescent and transmission images of individual infected fish at 5 dpi. Three independent fish injected with each strain are shown. Scale bar is 250 μm. Note the red autofluorescence of the yolk (Y). D. Live imaging of individual infected macrophages at 5 dpi in MmaM, RD1 and tesA::Tn embryos using fluorescence and DIC microscopy at high magnification (×600). The latter contain much lower bacterial loads.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Mol. Microbiol.