ZFIN ID: ZDB-FIG-110608-16
Quillien et al., 2011 - BMP signaling orchestrates photoreceptor specification in the zebrafish pineal gland in collaboration with Notch. Development (Cambridge, England)   138(11):2293-2302 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage Range: 10-13 somites to Prim-5

Fig. 4 BMP activity is necessary and sufficient for the expression of regulators of the PhR fate. (A-D′) Confocal sections of pineal glands, showing expression of neurod1 or otx5 (in red) and GFP (in green) from Tg(AANAT2:GFP) (A,A′,C,C′) and Tg(HuC:GFP) (B,B′,D,D′) transgenic embryos at 24 hours. White arrowheads indicate double-labeled cells. neurod1 and Tg(AANAT2:GFP) are co-expressed in the majority of cells, although single labeled cells are also present. The observation of neurod1+/Tg(AANAT2:GFP)- cells most probably reflects the earlier onset of expression for neurod1 compared with that of the Tg(AANAT2:GFP) transgene (Gothilf et al., 2002). Conversely, neurod1-/Tg(AANAT2:GFP)+ could be PhRs that do not express neurod1 during their life or alternatively more mature cells that have already turned off the gene. Similarly, although all Tg(AANAT2:GFP)+ cells are also otx5 positive, a number of single-labeled otx5+ cells are observed, which is probably due to the early onset of otx5 (see below) compared with the Tg(AANAT2:GFP) transgene. (E-L) Dorsal view of pineal glands stained for neurod1 (E-H) or otx5 (I-L) by in situ hybridization. Both genes start to be expressed at 16 hours. (M-O) Dorsal view of pineal gland from wild-type (M), Tg(hs:dnBmpr1a-CFP); Tg(hsp70:noggin3) double transgenic (N) and Tg(hs:bmp2b) embryos (O) at 24 hours stained for neurod1. (P) Quantification of the data represented in M-O. (Q-S) Dorsal view of pineal glands from wild-type (Q), Tg(hs:dnBmpr1a-CFP); Tg(hsp70:noggin3) double transgenic (R) and Tg(hs:bmp2b) embryos (S) at 24 hours stained for otx5. (T) Quantification of the data represented in Q-S. In M-T, Tg(hs:dnBmpr1a-CFP); Tg(hsp70:noggin3) transgenics were heat shocked at 16 hours, while Tg(hs:bmp2b) were heat shocked at 21 hours. The number (n) of embryos analyzed is noted for each case in P and T. Error bars represent s.d. ***P<0.0005 using a t-test. Anterior is upwards. Scale bars: 16 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP knu3Tg standard conditions Prim-5 epiphysis IFL
y8Tg standard conditions Prim-5 epiphysis IFL
neurod1 WT standard conditions 10-13 somites Not Detected epiphysis ISH
14-19 somites epiphysis ISH
20-25 somites epiphysis ISH
Prim-5 epiphysis ISH
fr13Tg heat shock Prim-5 epiphysis ISH
fr14Tg; ups4Tg heat shock Prim-5 Not Detected epiphysis ISH
knu3Tg standard conditions Prim-5 epiphysis ISH
y8Tg standard conditions Prim-5 epiphysis ISH
otx5 WT standard conditions 10-13 somites Not Detected epiphysis ISH
14-19 somites epiphysis ISH
20-25 somites epiphysis ISH
Prim-5 epiphysis ISH
fr13Tg heat shock Prim-5 epiphysis ISH
fr14Tg; ups4Tg heat shock Prim-5 epiphysis ISH
knu3Tg standard conditions Prim-5 epiphysis ISH
y8Tg standard conditions Prim-5 epiphysis ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development