FIGURE

Fig. 2

ID

ZDB-FIG-110411-6

Publication

Robles et al., 2011 - Characterization of genetically targeted neuron types in the zebrafish optic tectum

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Fig. 2

A majority of cells labeled in dlx5/6:GFP larvae are GABAergic. 3D reconstruction of a 5-dpf dlx5/6:GFP larvae that was fixed and sectioned at a thickness of 1 μm and labeled by GABA immunofluorescence and DAPI nuclear staining. (A) Colored lines indicate the relative locations of the cut-views presented in (B–D). (B) Cut-view in the XZ plane (dorsal view). (C) Cut-view in the YZ plane (sagittal). (D) Cut-view in the XY plane (coronal). Note the single-cell resolution in 3D and the high density of GABAergic cells in the periventricular cell layer (SPV) and neuropil (NP) regions of the tectum and the relative sparsity of GFP-labeled neurons. Arrows in (C) indicate axonal tracts of diencephalic and mesencephalic origins en route to the hindbrain. (E) A single section labeled with DAPI. Colored shading indicates the tectal regions defined for GABA phenotypic analysis: SPV (red), neuropil (blue), and superficial neuropil (green). (F) GABAergic phenotypic analysis in the optic tectum of 5 dpf dlx5/6:GFP larvae. Tegmentum (TG), hypothalamus (H), torus semicircularis (TS). Scale bar, 50 μm in (B–E).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Neural Circuits