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Fig. 4

ID

ZDB-FIG-110318-31

Publication

Felber et al., 2011 - Hedgehog signalling is required for perichondral osteoblast differentiation in zebrafish

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Fig. 4

Cyclopamine treatment downregulates expression of osteoblast markers predominantly in chondral ossification centres. (A, C, E, G and I) are control (EtOH treated) embryos and (B, D, F, H and J) are embryos treated with cyclopamine from 72 to 96 hpf. Expression of runx2a (A and B), runx2b (C and D), osx (E and F) and colX (G and H) was reduced or absent in the ceratohyal of cyclopamine treated fish. Expression in achondral ossification centres such as those of the dentary and maxilla was only mildly reduced or not affected at all. (I and J) shows ptc1 expression that shows that Hedgehog signalling is abolished in the perichodrium after cyclopamine treatment. (K) shown quantification of the staining shown in A–H. Please see Fig. 2 for abbreviations and notes.

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Acknowledgments

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Reprinted from Mechanisms of Development, 128(1-2), Felber, K., Croucher, P., and Roehl, H.H., Hedgehog signalling is required for perichondral osteoblast differentiation in zebrafish, 141-152, Copyright (2011) with permission from Elsevier. Full text @ Mech. Dev.