FIGURE

Fig. 7

ID
ZDB-FIG-110112-21
Publication
Lin et al., 2010 - Loss of Cofilin 1 Disturbs Actin Dynamics, Adhesion between Enveloping and Deep Cell Layers and Cell Movements during Gastrulation in Zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 7

Live cell imaging reveals loose cell-cell interactions between the DEL and EVL in cfl1 morphants.

Embryos injected with designated MOs and membrane-bound green fluorescent protein (GFP) mRNA, were dechorionated, immobilized, and examined by confocal microscopy for 10-min recordings at 10-s intervals per frame. The snapshots of representative embryos from the StdMO- (left column) or cfl1MO-injected embryos (right column) are shown. The recording times in minutes are denoted in the lower left corner of the left column. While StdMO-treated embryos showed continuous tight attachments between the DEL and EVL, DEL cells in cfl1 morphants did not form a tight connection with the EVL, which was further evidenced by the more-rounded cell shapes. Arrowheads indicate spaces between the EVL and DEL. A single-cell-thick EVL is to the right; yolk cells are toward the left; the animal pore is on the top; and the vegetal pore is at the bottom.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Shield to 75%-epiboly

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One