FIGURE

Fig. 5

ID
ZDB-FIG-101111-45
Publication
Prykhozhij, 2010 - In the Absence of Sonic Hedgehog, p53 Induces Apoptosis and Inhibits Retinal Cell Proliferation, Cell-Cycle Exit and Differentiation in Zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 5

Wild-type and shh-/- embryos at 24hpf (A-D) and 56 hpf (E, F) carrying p53 reporter transgene (PG13p21::nlsEGFP) were transversally sectioned, nuclei were stained with DAPI and apoptotic cells - with TUNEL, nlsEGFP signal is endogeneous (A-F). The developing nervous system of shh-/- mutant embryos showed high level of p53 reporter expression and apoptosis compared to wild-type embryos at both 24 (B-D) and 56 hpf (F). (A) At 24 hpf, p53 reporter was strongly induced in the shh-/- mutant compared to the wild-type retina, but there was no elevated apoptosis in the shh-/- mutant retina. (B) Expression of p53 reporter and TUNEL labeling in midbrain of shh-/- mutant and wild-type embryos at 24 hpf. (C) Expression of p53 reporter and TUNEL labeling in hindbrain of shh-/- mutant and wild-type embryos at 24 hpf. (D) Expression of p53 reporter and TUNEL labelling in spinal cord of shh-/- and wild-type at 24 hpf. (E) At 56 hpf, both p53 reporter expression and apoptosis were much higher in the shh-/- retina than in the wild-type retina. (F) Expression of p53 reporter and TUNEL labelling in hindbrain regions of shh-/- and wild-type embryos at 56 hpf.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One