FIGURE
Fig. 7
- ID
- ZDB-FIG-100628-68
- Publication
- Kim et al., 2010 - Gli2a protein localization reveals a role for Iguana/DZIP1 in primary ciliogenesis and a dependence of Hedgehog signal transduction on primary cilia in the zebrafish
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Fig. 7
GFP-tagged Iguana (Igu) localizes to basal bodies of primary cilia in zebrafish embryos. GFP-igu mRNA was injected into 1-cell stage embryos and injected embryos fixed and labelled with anti-acetylated tubulin (red). Punctate GFP-Igu localization was excluded from the axoneme (a). Co-injection of mRNA encoding tdTomato fused GTAP3 (γ-tubulin associated protein 3) and GFP-Igu, revealed co-localization of both proteins at the basal bodies in live early somitogenesis stage embryos (a′). The basal body localization of GFP-Igu was un-altered in embryos expressing dnPKA (b). Scale bars: 5 μm |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
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Full text @ BMC Biol.