FIGURE

Fig. 2

ID
ZDB-FIG-100506-56
Publication
Gauthier et al., 2010 - De novo mutations in the gene encoding the synaptic scaffolding protein SHANK3 in patients ascertained for schizophrenia
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2

Validation of Shank3 mutations in zebrafish. Knockdown (KD) of either of the zebrafish Shank3 genes (zs3.1, zs3.2) using selective AMOs resulted in severe morphological (A) and behavioral deficits (B) compared with wild type (WT), as illustrated using representative images taken from high-speed video films. Partial rescue was observed with coinjection of AMO and rat WT Shank3 mRNA. The pie charts depict the proportion (% of totals) of normal (control-like; yellow), severely affected (no swimming; red), and mildly affected (slow swimming; blue) embryos in each group. The results with coinjection of AMO and rat WT Shank3 or mutated (R1117X or R536W) mRNA are summarized in bar graphs (C). The asterisk denotes significant (P < 0.001) differences from the KD group.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.
Open Access.
Full text @ Proc. Natl. Acad. Sci. USA
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.