FIGURE

Fig. S4

ID

ZDB-FIG-100504-62

Publication

Hartnett et al., 2010 - Insulin-like growth factor-2 regulates early neural and cardiovascular system development in zebrafish embryos

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Fig. S4

IGF-2a and IGF-2b are required for the development of anterior neural structures during zebrafish gastrulation and play anti-apoptotic roles during segmentation. (A, B) Knockdown of igf-2a or igf-2b results in a reduction of pax6.2 expression in the developing eye and midline region. (C, D) Knockdown of igf-2a or igf-2b causes a reduction of rx3 expression. (E) Knockdown of igf-2a results in an increase in apoptosis, particularly in the developing spinal cord. (F) Knockdown of igf-2b results in an increase in apoptosis, particularly in the anterior region of the embryo. Frequency of embryos displaying this staining pattern; A, 14/32; B, 8/20; C, 15/39; D, 14/33; E, 17/30; F, 12/33. A-D are views of the future anterior region and E, F are lateral views.

Expression Data

Genes:	pax6b rx3
Fish:	AB + MO1-igf2a AB + MO3-igf2b
Knockdown Reagents:	MO1-igf2a MO3-igf2b
Anatomical Terms:	axis forebrain neural keel optic primordium
Stage:	Bud

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-igf2a AB + MO3-igf2b
Knockdown Reagents:	MO1-igf2a MO3-igf2b
Observed In:	apoptotic process neural keel spinal cord neural keel
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Dev. Biol.