Six3b depletion rescues prethalamic patterning defects in Zic2a-depleted embryos. (A) Uninjected embryos show normal dlx2a expression in the diencephalon (29/29 embryos, n=2). (B) zic2a morphants exhibit a strong reduction of diencephlic dlx2a (18/19 embryos, n=2). (C) six3b morphants show normal dlx2a patterning (23/23 embryos, n=2). (D) Embryos co-injected with six3bMO and zic2aMO exhibit partially rescued prethalamic dlx2a expression (30/52 embryos, n=2). (E) A graphic summary of the dlx2a rescue experiments. (F) Normal arx expression is observed in the PT of uninjected control embryos. (G) arx expression is strongly reduced in zic2a morphants (13/15 embryos, n=2). (H) six3b morphants show normal arx expression. (I) Embryos co-injected with six3bMO and zic2aMO show partial rescue of arx (10/23 embryos, n=2). (J) A graphic summary of the arx domain rescue experiments. Red, strongly reduced dlx2a or arx in PT; blue, normal or nearly normal expression of dlx2a or arx in PT. Arrowheads point to the diencephalic domains of dlx2a or arx expression. Lateral views with anterior to the left shown at prim-5 (A-D) or 20s (F-I).
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