FIGURE

Fig. 1

ID
ZDB-FIG-090904-40
Publication
Jing et al., 2009 - Zebrafish ale oko, an essential determinant of sensory neuron survival and the polarity of retinal radial glia, encodes the p50 subunit of dynactin
Other Figures
All Figure Page
Back to All Figure Page
Fig. 1

ale oko phenotype in the zebrafish retina. (A-B′) External phenotypes of akojj50 mutants (A′,B′), compared with their wild-type siblings (A,B). Dorsal (A,A′) and lateral (B,B′) views are shown at 5 dpf. (C-D′) Transverse sections of the ako (C′,D′) and wild-type (C,D) retinae at 4 dpf. (E,E′) Electron micrographs of wild-type (E) and mutant (E′) retinae at 3 dpf. OS, outer segment; IS, inner segment. Asterisks indicate abnormal mutant outer segments. (F-K′) Differentiation of retinal cells classes in ako mutants. Transverse cryosections through retinae of wild type (top) and akojj50 mutants (bottom). (F-G) Müller glia are visualized via the expression of a GFP transgene (F,F′, green, arrows) or by antibody staining against carbonic anhydrase (G,G′, red). Photoreceptors are stained with the Zpr-1 antibody (red in F,F′, green in G,G′). (H-J′) Transverse sections stained with antibodies to subpopulations of amacrine cells: anti-serotonin (H,H′, green), anti-neuropeptide Y (I,I′, red, arrows), anti-parvalbumin (I,I′, green) and anti-GABA (J,J′). (K,K) Sections through retinae of Tg(brn3c:mGFP) transgenic animals, which express GFP in ganglion cells (green). Photoreceptors are visualized with the Zpr-1 antibody (red). In G-K′, asterisks indicate the optic nerve. L, lens; rpe, retinal pigment epithelium; prcl, photoreceptor cell layer; inl, inner nuclear layer; ipl, inner plexiform layer; gcl, ganglion cell layer. Scale bars: 1 mm in A-B′; 40 μm in C,C′,F-K′; 30 μm in D,D′; 5 μm in E,E′.

Expression Data
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Pec-fin to Day 4

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: Pec-fin to Day 5

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development