FIGURE

Fig. 4

ID

ZDB-FIG-090623-23

Publication

Ma et al., 2009 - Mosaic hoxb4a neuronal pleiotropism in zebrafish caudal hindbrain

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Fig. 4

Hoxb4a activity along the dorsoventral axis in r7–8.

(A–J) Optically reconstructed transverse sections at the level of r7 (A, C, E, G, I) and r8 (B, D, F, H, J) in 1, 2, 4, 20 and 30 dpf transgenic zebrafish. Dashed lines outline the hindbrain boundaries in the sections with anterior-posterior position indicated in Fig. 4 (A–E). (K–L) Single plane images acquired from the ventral side of an isolated brain at 40 μm (K) and 70 μm (L) depth showing hoxb4a-YFP expression and reticulospinal neurons from r7 to rostral r8 at 4 dpf. Arrowheads point to r8-reticular neurons that expressed hoxb4a-YFP. Inset in L is an overview of the caudal reticular scaffold and hoxb4a cells imaged from the ventral side with the box indicating the viewing field in K–L. Abbreviations: Ant, anterior. Ca, Ca neuron. Llf, lateral-longitudinal fasciculus. Mlf, medial-longitudinal fasciculus. r8-Ret, r8-reticular neurons. Scale bars = 50 μm (A–J) and 10 μm (K–L).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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