Fig. 6

Ethanol treatment impairs migration of prechordal plate cells. (A to D) Control embryo (A) and ethanol-treated embryos (B to D) at the 100% epiboly stage. Embryos were simultaneously stained with gsc (g) and pax-2 (p) probes. In most cases, gsc-expressing cells formed a cluster which was retarded in its anterior migration (B, C). Less frequently, the gsc expression domain (g) was split in the ethanol-treated embryo (D). (E to H) Control and ethanol-treated embryos stained with gsc and pax-2 antisense probes (blue) and anti-Ntl antibody (brown). The expression domains of gsc (g), pax-2 (p), and Ntl (N) are indicated; gsc expression in G is out of the plane of focus. Open triangles in F and H indicate planes of sections shown in Figs. 7B and 7A, respectively. Orientation of embryos is anterior up and view is from the dorsal aspect.