FIGURE

Fig. 2

ID

ZDB-FIG-090304-2

Publication

Slanchev et al., 2009 - Control of Dead end localization and activity – Implications for the function of the protein in antagonizing miRNA function

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Fig. 2

Deletions of parts of Dead end protein reveal domains important for protein localization and function. (A) Schematic drawing of Dead end deletion constructs. (B) Intracellular localization of the various Dnd-deletion fusion proteins detected by immunohistochemistry with anti-HA antibody (Green) in comparison with the position of the GCGs reported by anti-vasa antibody staining (Red). (C) A chart depicting the ability of the various deletions constructs to supplement for wild type Dead end. The values represent the number of rescued PGCs in embryos co-injected with a specific Dnd truncated protein and dnd MO, normalized to the PGCs rescued by a wild type dnd mRNA injection (see the text for details).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-dnd1
Knockdown Reagent:	MO1-dnd1
Observed In:	primordial germ cell
Stage:	Unknown

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 126(3-4), Slanchev, K., Stebler, J., Goudarzi, M., Cojocaru, V., Weidinger, G., and Raz, E., Control of Dead end localization and activity – Implications for the function of the protein in antagonizing miRNA function, 270-277, Copyright (2009) with permission from Elsevier. Full text @ Mech. Dev.