FIGURE

Fig. 1

ID

ZDB-FIG-081107-30

Publication

Krishnan et al., 2008 - Identification of Spt5 target genes in zebrafish development reveals its dual activity in vivo

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Fig. 1

Spt5 protein is significantly decreased in fog^sk8 embryos.

(A–B) Whole mount immunofluorescence detection of Spt5 and RNAPII in zebrafish embryos, showing the nuclear localization pattern of Spt5 protein. (C–D) Whole mount immunofluorescence detection of Spt5 in WT and fog^sk8 embryos at 24 hpf, showing overall decrease of Spt5 protein in fog^sk8 embryos. Confocal images of lateral views are shown, with the anterior to the left and dorsal up. (E) Western blot of wild-type (WT) and fog^sk8 embryonic extracts at 19 hpf and 24 hpf shows substantial decrease in maternal Spt5 protein in the mutant. (F–G) Light microscopic view of WT and sk8 embryos at 24 hpf, showing the gross morphological phenotype of the mutant embryos.

Expression Data

Genes:	polr2a supt5h
Antibody:	Ab1-polr2a
Fish:	AB supt5h^sk8/sk8
Anatomical Terms:	brain optic cup whole organism whole organism nucleus
Stage Range:	Bud to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	supt5h^sk8/sk8
Observed In:	brain extension optic cup post-vent region
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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