ZFIN ID: ZDB-FIG-081104-16
Spoorendonk et al., 2008 - Retinoic acid and Cyp26b1 are critical regulators of osteogenesis in the axial skeleton. Development (Cambridge, England)   135(22):3765-3774 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Observed In:
Stage: Days 7-13

Fig. 2 stocksteif encodes cyp26b1. (A) stocksteif was mapped to a narrow region on linkage group 7. Flanking SSLP markers are both located on BAC zC197C14. cyp26b1 appears to be the only gene present on the clone. (B) Schematic overview of cyp26b1 structure on the genomic level with nonsense (K46STOP or sstsa0002) and splice donor (sstsa0003) mutation indicated. Translated parts of the exons are shaded. (C,D) Alizarin Red bone staining of a cyp26b1+/+ embryo and a transheterozygous cyp26b1(sstsa0002/sstsa0003) embryo. (C′,D′,E-G) Double staining with Alcian Blue for cartilage and Alizarin Red for bone. (C′,D′) A cyp26b1+/+ embryo shows normal cartilage, whereas a transheterozygous cyp26b1(sstsa0002/sstsa0003) embryo shows missing tissue dorsal to the ethmoid plate (asterisk) and fusion of the ceratohyal with the basihyal (arrow). (E-G) Ventral views of a wild-type embryo (E), a homozygous sstt24295 mutant (F) and a homozygous sstsa0002 mutant (G). Note the reduced and misshaped ethmoid plate (asterisk) in the sstsa0002 mutant. (H-K) Expression of col10a1 is not changed in 4 dpf wild-type siblings (H,I) versus mutants (J,K). cl, cleithrum; en, entopterygoid; op, operculum; ps, parasphenoid.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
col10a1a WT standard conditions Day 4 cleithrum ISH
Day 4 entopterygoid ISH
Day 4 opercle ISH
Day 4 parasphenoid ISH
cyp26b1sa2/sa2 standard conditions Day 4 cleithrum ISH
Day 4 entopterygoid ISH
Day 4 opercle ISH
Day 4 parasphenoid ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
cyp26b1sa2/+; cyp26b1sa3/+ standard conditions Days 7-13 basihyal cartilage malformed, abnormal
Days 7-13 ceratohyal cartilage malformed, abnormal
Days 7-13 ethmoid cartilage malformed, abnormal
cyp26b1sa2/sa2 standard conditions Days 7-13 ceratohyal cartilage malformed, abnormal
Days 7-13 ethmoid cartilage decreased size, abnormal
Days 7-13 ethmoid cartilage malformed, abnormal
cyp26b1t24295/t24295 standard conditions Days 7-13 ceratohyal cartilage malformed, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development