ZFIN ID: ZDB-FIG-081007-44
Russek-Blum et al., 2008 - Dopaminergic neuronal cluster size is determined during early forebrain patterning. Development (Cambridge, England)   135(20):3401-3413 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec

Fig. S2 All TH-positive DA neurons express neurog1 in the diencephalon. (A-A′″) Lateral view of neurog1::gfp transgenic reporter embryo (at 24 hpf), which was subjected to in situ hybridization with an antisense barhl2 probe followed by double immunofluorescence staining with antibodies against TH and GFP (A). High-magnification images of cells expressing neurog1, TH and barhl2 are shown in A′-A′″, respectively. Arrows indicate neurog1+ TH+ double-positive cells. (B-B′″) Dorsal view of double-transgenic neurog1::nRFP; dlx6a::GFP embryo (at 48 hpf), which was subjected to immunofluorescence staining with antibodies against TH (B). Arrowheads indicate neurog1+ TH+ double-positive neurons. Single-channel fluorescent images are presented in B′-B′″. PT, posterior tuberculum; RFP, red fluorescent protein; Tel, telencephalon. Scale bars: 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
barhl2 sb1Tg standard conditions Prim-5 diencephalon ISH
GFP ot1Tg; sb3Tg standard conditions Long-pec caudal tuberculum IHC
Long-pec diencephalon IHC
sb1Tg standard conditions Prim-5 caudal tuberculum IHC
Prim-5 caudal tuberculum dopaminergic neuron IHC
Prim-5 diencephalon IHC
Prim-5 telencephalon IHC
RFP ot1Tg; sb3Tg standard conditions Long-pec caudal tuberculum dopaminergic neuron IHC
th ot1Tg; sb3Tg standard conditions Long-pec caudal tuberculum dopaminergic neuron IHC
sb1Tg standard conditions Prim-5 caudal tuberculum dopaminergic neuron IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development