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Fig. 3

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ZDB-FIG-080916-12

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Halbig et al., 2008 - Zebrafish U6 small nuclear RNA gene promoters contain a SPH element in an unusual location

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Fig. 3

In vitro transcription of zebrafish U6 wt or mutant promoter gene plasmids. A 100 μg aliquot of HeLa S100 extract was incubated with 1 μg of pGEM vector, hU6-1maxi, zU6-1maxi, TATATMUT promoter, or SPHMUT promoter plasmid DNAs, and included nucleoside triphosphates (with radiolabeled α-GTP) and α-amanitin at a final concentration of 2 μg/mL. Nucleic acids were purified and electrophoresed on a 10% polyacrylamide/8.3 M urea gel. “U6maxi” represents specific transcripts from U6 promoters. “tRNA^his” shows guanylyltransferase labeling of endogenous tRNA in the S100 extract and serves as a recovery and normalization control for quantification of each sample. Numbers in the row below the lane numbers are the relative percentage amounts of normalized zU6-1maxi transcript compared to the wt promoter (lane 3), and are the average of two independent reactions. Numbers in parentheses are the relative transcription levels from each experiment.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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Reprinted from Gene, 421(1-2), Halbig, K.M., Lekven, A.C., and Kunkel, G.R., Zebrafish U6 small nuclear RNA gene promoters contain a SPH element in an unusual location, 89-94, Copyright (2008) with permission from Elsevier. Full text @ Gene