PUBLICATION

Zebrafish U6 small nuclear RNA gene promoters contain a SPH element in an unusual location

Authors
Halbig, K.M., Lekven, A.C., and Kunkel, G.R.
ID
ZDB-PUB-080722-2
Date
2008
Source
Gene   421(1-2): 89-94 (Journal)
Registered Authors
Lekven, Arne
Keywords
eukaryotic transcription, snRNA gene transcription, transcription element, SBF/Staf/ZNF143
MeSH Terms
  • 5' Flanking Region
  • Animals
  • Base Sequence
  • Consensus Sequence
  • Humans
  • Molecular Sequence Data
  • Promoter Regions, Genetic*
  • RNA, Small Nuclear/biosynthesis
  • RNA, Small Nuclear/genetics*
  • Sequence Alignment
  • Trans-Activators/metabolism
  • Transcription, Genetic
  • Zebrafish/genetics*
PubMed
18619527 Full text @ Gene
Abstract
Promoters for vertebrate small nuclear RNA (snRNA) genes contain a relatively simple array of transcriptional control elements, divided into proximal and distal regions. Most of these genes are transcribed by RNA polymerase II (e.g., U1, U2), whereas the U6 gene is transcribed by RNA polymerase III. Previously identified vertebrate U6 snRNA gene promoters consist of a proximal sequence element (PSE) and TATA element in the proximal region, plus a distal region with octamer (OCT) and SphI postoctamer homology (SPH) elements. We have found that zebrafish U6 snRNA promoters contain the SPH element in a novel proximal position immediately upstream of the TATA element. The zebrafish SPH element is recognized by SPH-binding factor/selenocysteine tRNA gene transcription activating factor/zinc finger protein 143 (SBF/Staf/ZNF143) in vitro. Furthermore, a zebrafish U6 promoter with a defective SPH element is inefficiently transcribed when injected into embryos.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping