FIGURE

Fig. S3

ID

ZDB-FIG-080904-59

Publication

Kai et al., 2008 - Sphingosine-1-phosphate receptors regulate individual cell behaviours underlying the directed migration of prechordal plate progenitor cells during zebrafish gastrulation

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Fig. S3

Abrogation of Mil and Edg1 function modulates movements of the presumptive hypothalamus. (A-D) Uninjected WT embryo (A) and WT embryos injected with 4.3 ng mil-MO (B), 6.2 ng edg1-MO (C) or 4.3 ng mil-MO + 6.2 ng edg1-MO (D). The embryos were fixed at tailbud stage for in situ hybridisation with the markers dlx3 (for the anterior edge of the neural plate), pax2.1 (for the prospective mid-hind brain boundary) and nkx2.1 (for the presumptive hypothalamus). The position of the presumptive hypothalamus is highlighted by the dotted lines.

Expression Data

Genes:	dlx3b nkx2.4b pax2a
Fish:	WT WT + MO1-s1pr1 WT + MO1-s1pr2 WT + MO1-s1pr1 + MO1-s1pr2
Knockdown Reagents:	MO1-s1pr1 MO1-s1pr2
Anatomical Terms:	anterior neural plate midbrain hindbrain boundary neural keel neural keel
Stage:	Bud

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-s1pr1 WT + MO1-s1pr2 WT + MO1-s1pr1 + MO1-s1pr2
Knockdown Reagents:	MO1-s1pr1 MO1-s1pr2
Observed In:	cell migration involved in gastrulation neural keel
Stage:	Bud

Phenotype Detail

Acknowledgments

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