bucky ball is required to localize vegetal pole and restrict animal pole localized oocyte transcripts. Animal pole localization of pou2 in in situ hybridizations on sections of wild-type stage II (A) and stage IV (B) oocytes. (C) In situ hybridizations on sections show pou2 enriched at multiple cortical sites in buc stage II oocytes, and panel D radially expanded at the cortex in buc stage IV oocytes. (E) Animal pole (AP) view of pou2 localization in a wild-type stage IV oocyte. (F) In buc mutant oocytes pou2 transcripts are not asymmetrically localized. (G) Vg1 localization (arrow) at the animal pole beneath the micropyle in wild-type, and panel H in multiple, ectopic domains in buc mutant oocytes. In situ hybridization on sections reveals brl enrichment at the vegetal cortex (arrowhead) in wild-type early stage II (I) and late stage II (J) oocytes. At stage III of oogenesis, brl transcripts are discretely localized to the vegetal cortex in wild-type (K). In stage II bucky ball mutant oocytes, brl remains throughout the oocyte (L, M) and is also not localized in stage III (N) oocytes. (O) mago nashi transcripts localize to the vegetal pole in wild-type stage III oocytes (arrowhead). (P) Although present in primary oocytes (arrow, also in panel O), mago nashi is not detectable by in situ hybridization in stage III buc oocytes. (A–D, I–N) Hybridizations on slides containing 10 μM ovary sections. (E–H and O–P) Whole mount in situ hybridization images. Stage is shown in the lower, left of each panel. Bars are 50 μm.
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