FIGURE

Fig. 7

ID

ZDB-FIG-080904-17

Publication

Sperber et al., 2008 - barx1 is necessary for ectomesenchyme proliferation and osteochondroprogenitor condensation in the zebrafish pharyngeal arches

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Fig. 7

Barx1 positively influences cellular proliferation within the pharyngeal arches. (A, B) Sagittal serial sections (10 μm) of the pharyngeal arch region stained with Phosphohistone-H3 antibody. (A) Control embryo at 48 hpf. (B) barx1 morphant. The neural crest derived mesenchyme is overlaid in green; arrows indicate stained nuclei; e, eye; m, mandibular arch; h, hyoid arch; gill arches are numbered. Anterior is to the left. Scale bar: 50 μm. (C) Averaged cell proliferation in control uninjected embryos (black bars) and barx1 morphants (see Fig. 4; white bars) was determined within the mesenchyme as shown in panels A, B at the indicated four time points. Approximately fifty serial sections per embryo were examined. Number of embryos examined is indicated above each bar. Scale bar: (A, B) 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	EKW + MO1-barx1 + MO2-barx1
Knockdown Reagents:	MO1-barx1 MO2-barx1
Observed In:	cartilage development cell population proliferation pharyngeal arch mesenchyme
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 321(1), Sperber, S.M., and Dawid, I.B., barx1 is necessary for ectomesenchyme proliferation and osteochondroprogenitor condensation in the zebrafish pharyngeal arches, 101-110, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.