FIGURE

Fig. 3

ID

ZDB-FIG-080620-37

Publication

Fadool et al., 1998 - Transposition of the mariner element from Drosophila mauritiana in zebrafish

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Fig. 3

PCR demonstration of inheritance of the mariner element without plasmid vector sequences. (a) Genomic DNA isolated from individual F₂ larvae was tested for the presence of the mariner element and flanking plasmid sequences. Amplification of mariner sequences identified 11 of 16 (68%) larvae as positive for the transgene (arrow) (lane M, 100-bp marker, Promega; lane B, no DNA control). (b) Amplification with one primer specific for mariner and one for the M13 reverse sequence flanking the insert in the pWE1 plasmid revealed none possessed this plasmid DNA. The control lane (C), containing 1 ng pWE1 plasmid, demonstrated the expected amplification product of 680 bp (arrowhead).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA