ZFIN Image: Fadool et al., 1998, Fig. 3

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Figure Caption

Fig. 3 PCR demonstration of inheritance of the mariner element without plasmid vector sequences. (a) Genomic DNA isolated from individual F₂ larvae was tested for the presence of the mariner element and flanking plasmid sequences. Amplification of mariner sequences identified 11 of 16 (68%) larvae as positive for the transgene (arrow) (lane M, 100-bp marker, Promega; lane B, no DNA control). (b) Amplification with one primer specific for mariner and one for the M13 reverse sequence flanking the insert in the pWE1 plasmid revealed none possessed this plasmid DNA. The control lane (C), containing 1 ng pWE1 plasmid, demonstrated the expected amplification product of 680 bp (arrowhead).

Acknowledgments

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