FIGURE

Fig. S7

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ZDB-FIG-080605-7

Publication

Sidi et al., 2008 - Chk1 Suppresses a Caspase-2 Apoptotic Response to DNA Damage that Bypasses p53, Bcl-2, and Caspase-3

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Fig. S7

Chk1-suppressed apoptosis occurs predominantly during the interphase in zebrafish embryos
(A) Confocal images of the caudal spinal cord in representative embryos of each indicated genotype processed for TUNEL (green)/pH3 (red) double-labeling. While approximately half of the pH3-labeled cells in irradiated p53^e7/e7;chk1^MO embryos are TUNEL positive (see panel B), the bulk of TUNEL-stained nuclei are pH3 negative, implying that death mainly occurs during the interphase. By contrast, cisplatin- or doxorubicin-induced death of MK-2–depleted Tp53^-/- MEFs occurs exclusively in mitosis (Reinhardt et al., 2007).
(B) Percentage of double-labeled cells among >200 pH3-positive cells visualized on images as in panel A. Data are means ± SEM.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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Reprinted from Cell, 133(5), Sidi, S., Sanda, T., Kennedy, R.D., Hagen, A.T., Jette, C.A., Hoffmans, R., Pascual, J., Imamura, S., Kishi, S., Amatruda, J.F., Kanki, J.P., Green, D.R., D'Andrea, A.A., and Look, A.T., Chk1 Suppresses a Caspase-2 Apoptotic Response to DNA Damage that Bypasses p53, Bcl-2, and Caspase-3, 864-877, Copyright (2008) with permission from Elsevier. Full text @ Cell