FIGURE

Fig. 6

ID

ZDB-FIG-080418-32

Publication

Kinsey et al., 2003 - Activation of Src-family PTK activity at fertilization: role of the SH2 domain

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Fig. 6

Effect of GST-Fyn-SH2 on Src-family PTK activity in vitro. Immunoprecipitates prepared from the particulate fraction of solubilized zebrafish zygotes (5 min postinsemination) using the Pan-Src antibody (Src-2) to pull down Src family kinases. Kinase activity in the immunoprecipitates was measured as described in materials and methods. Kinase reactions contained either no addition (A) or 1 μM (B), 4 μM (C), or 8 μM GST-FYN-SH2 fusion protein (D) to test the effect of this fusion protein on kinase activity in vitro. The position of the autophosphorylated Src-family kinase band is indicated by the arrow.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 264(1), Kinsey, W.H., Wu, W., and Macgregor, E., Activation of Src-family PTK activity at fertilization: role of the SH2 domain, 255-262, Copyright (2003) with permission from Elsevier. Full text @ Dev. Biol.