PUBLICATION
Activation of Src-family PTK activity at fertilization: role of the SH2 domain
- Authors
- Kinsey, W.H., Wu, W., and Macgregor, E.
- ID
- ZDB-PUB-031119-4
- Date
- 2003
- Source
- Developmental Biology 264(1): 255-262 (Journal)
- Registered Authors
- Kinsey, William H., Wu, Wen-Jun
- Keywords
- none
- MeSH Terms
-
- Animals
- Calcium/metabolism
- Enzyme Activation
- Fertilization/physiology*
- Glutathione Transferase/genetics
- Glutathione Transferase/metabolism
- Male
- Oocytes/cytology
- Oocytes/physiology
- Proto-Oncogene Proteins/genetics
- Proto-Oncogene Proteins/metabolism
- Proto-Oncogene Proteins c-fyn
- Recombinant Fusion Proteins/genetics
- Recombinant Fusion Proteins/metabolism*
- Spermatozoa/metabolism
- Zebrafish
- Zebrafish Proteins
- src Homology Domains*
- src-Family Kinases/genetics
- src-Family Kinases/metabolism*
- PubMed
- 14623246 Full text @ Dev. Biol.
Citation
Kinsey, W.H., Wu, W., and Macgregor, E. (2003) Activation of Src-family PTK activity at fertilization: role of the SH2 domain. Developmental Biology. 264(1):255-262.
Abstract
The role of Src-family protein tyrosine kinases (SFKs) in egg activation has been established, in large part, by the observation that GST fusion proteins encoding the SH2 domain of Src or Fyn suppress the sperm-induced calcium transient and cause polyspermy in marine invertebrate eggs. These fusion proteins are thought to act as dominant-negative inhibitors of SFK function; however, the mechanism by which they work is not known. The objective of the present study was to test the hypothesis that fusion proteins containing the above SH2 domains prevent activation of SFKs in response to fertilization. A single cell assay was developed that allows estimation of SFK activity in eggs injected with the GST-Fyn-SH2 fusion protein. The results demonstrate that the GST-Fyn-SH2 fusion protein prevents fertilization induced stimulation of SFK activity at concentrations that also suppress the sperm-induced calcium transient in zebrafish eggs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping