Whole-mount in situ hybridization for emilin3a and emilin3b in zebrafish embryos at different developmental stages. A-I: In situ hybridization was performed with probes for emilin3a (A-C,G,H) or emilin3b (D-F,I). A: Expression of emilin3a in the notochord and in the chordoneural hinge (asterisk) of the tail bud at 15 somites (s). B: strong labeling of the notochord by emilin3a at 20 s. C: At 24 hours postfertilization (hpf), expression of emilin3a decreases in the notochord and becomes evident in the floor plate of developing spinal cord and in the most posterior part of the hypochord. D: Labeling of the notochord by emilin3b at 15 s. E: Higher magnification of the posterior region of a 15 s embryo, showing emilin3b expression in the notochord and in the chordoneural hinge. F: At 24 hpf, expression of emilin3b in the notochord decreases and shows faint labeling of some cells in the floor plate and hypochord. G,H: Expression of emilin3a in the cartilage of branchial arches (G) and of craniofacial elements (H) at 48 hpf. I: Labeling of craniofacial mesenchyme by emilin3b at 48 hpf. Except for A and D, embryos were dissected away from the yolk and mounted between glass coverslips. A-C,E-G are lateral view, anterior side of embryo is on the left; D,H,I are dorsal view, anterior side is on the left in D and at the top in H and I. ba1-5, first-fifth branchial arches; cnh, chordoneural hinge; ep, ethmoid plate; fp, floor plate; h, hyoid cartilage; hy, hypochord; m, mandibular cartilage; no, notochord; o, opercular cartilage; tc, trabeculae cranii; yo, yolk
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