FIGURE

Fig. 4

ID
ZDB-FIG-080326-51
Publication
Bedell et al., 2005 - roundabout4 is essential for angiogenesis in vivo
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Fig. 4

robo4 gene knockdown affects flk+ ISV and rescue of defects by hrobo4. Splice MOs alone (12 ng) or in combination with hrobo4 RNA (150 pg) were microinjected into Tg(vegfr2: G-RCFP)y10 zebrafish embryos at the one-cell stage, and images were taken at 22-24 som and 48 hpf. From left to right, images are MO, msMO, and MO+hrobo4-injected embryos. A-C and G-I show embryos at 22-24 som, whereas D-F and J-L show embryos at 48 hpf. (A and D) MO1 (n = 31). (G and J) MO2 (n = 28). (B and E) msMO1 (n = 31). (H and K) msMO2 (n = 54). (C and F) MO1+hrobo4 (n = 39). (I and L) MO2+hrobo4 (n = 46). (M) Quantitation for rescue experiments with hrobo4 (A-L). (N) Quantitation of embryos injected for WT (n = 124), MO2 (n = 119), MO2+robo1 (n = 82), MO2+robo2 (n = 115), and MO2+robo4 (n = 91). (O) Quantitation comparison of overexpression experiment for WT (n = 118), hrobo4 (n = 71), and robo4 (n = 91) RNA-injected (150 pg) embryos. A defective embryo was counted as three or more missing ISV. Anterior is to the left.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA