FIGURE

Fig. 4

ID
ZDB-FIG-080325-34
Publication
Monteiro et al., 2008 - Two novel type II receptors mediate BMP signalling and are required to establish left-right asymmetry in zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

Overexpression of dominant negative bmpr2a and bmpr2b block BMP signalling in vivo. Unless otherwise stated, bmpr2a-Δic and bmpr2b-Δic mRNA were injected at 100 ng/μl. (A) Smad-dependent BMP signalling upon overexpression of full length or truncated bmpr2a and bmpr2b mRNA in embryos. Injection of 200 ng/μl of bmpr2a-Δic or bmpr2b-Δic mRNA efficiently blocked endogenous BRE-Luciferase activity in embryos at 70% epiboly. Injection of mRNA encoding tBR, a Xenopus dominant negative ALK3, was injected as a control. (B–D) Gata1 expression at 12 somites in wild type (B), bmpr2a-Δic (C) and bmpr2b-Δic (D) injected embryos. (E) At the 4-somite stage, pax2 is expressed in the midbrain–hindbrain boundary (mhb), otic placodes (ot) and pronephric (ventral) mesoderm (p), while myoD is expressed in the presomitic (axial) mesoderm (m) in wild type embryos. Embryos injected with bmpr2a-Δic (F) or bmpr2b-Δic (G) showed an expanded pax2 expression in the mhb domain concomitant with a strong reduction of pax2 expression in the otic placodes. (H) Expression of myoD in the somites of a wild type 14-somite stage embryo (lateral view, anterior to the right). Overexpression of bmpr2a-Δic (I) or bmpr2b-Δic (J) in 14-somite stage embryos. Note that the myoD expression is ventrally expanded, surrounding the most posterior end of the defective axis; the most anterior somites do not seem to be affected. RLU - Relative Luciferase Units.

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage Range: 1-4 somites to 14-19 somites

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 315(1), Monteiro, R., van Dinther, M., Bakkers, J., Wilkinson, R., Patient, R., Ten Dijke, P., and Mummery, C., Two novel type II receptors mediate BMP signalling and are required to establish left-right asymmetry in zebrafish, 55-71, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.