FIGURE

Fig. S3

ID

ZDB-FIG-071223-17

Publication

White et al., 2007 - Complex Regulation of cyp26a1 Creates a Robust Retinoic Acid Gradient in the Zebrafish Embryo

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Fig. S3

Global RA Treatments Rescue Hindbrain Segmentation over a Wide Range of Concentrations. Embryos were treated at 4 hpf with DEAB either with or without a low concentration of RA between 0.625–20 nM, fixed at 19 hpf, and pattern was assessed by in situ hybridization for hoxd4a (blue; r6/7 boundary), krox-20 (egr2b, red; r3 and r5), and pax2a (red; midbrain–hindbrain boundary). (A) Wild-type expression. (B) DEAB-treated embryos show loss of r5–r7. (C–F) Concentrations from 0.625–5 nM rescue wild-type patterning to the same extent. (G–H) Higher concentrations produce anterior defects.

Expression Data

Genes:	egr2b hoxd4a pax2a
Fish:	WT
Conditions:	chemical treatment: 4-(diethylamino)benzaldehyde chemical treatment: 4-(diethylamino)benzaldehyde
Anatomical Terms:	midbrain hindbrain boundary rhombomere 3 rhombomere 5 rhombomere 7
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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