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Fig. 9

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ZDB-FIG-070920-28

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Sheela et al., 2005 - Zebrafish ftz-f1a (nuclear receptor 5a2) functions in skeletal muscle organization

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Fig. 9

Injection of ff1a into yot mutants results in increased fast muscle myofibrils. The yot mutants were injected with (A, C, F) control β-gal or (B, D, G) ff1a RNA followed by staining with (A–D) F59 or (F, G) S58 plus zm4 antibodies at 24 hpf, and somites 14–17 are shown in lateral view with anterior towards the left. (A) The yot mutant reacted very weakly with F59 antibody at 24 hpf. (B) Ectopic expression of ff1a in yot mutants results in increased F59 staining. (C–G) Cross-sections. (C, D) The location of F59 staining (red) in the myotome indicates that these are fast muscles. Nuclei are stained with Hoechst dye (blue). (E) Slow muscle cells of normal siblings (WT) stained with S58 are arranged as a monolayer at the periphery of the somite, and fast muscle cells stained by zm4 are located more medially at 24 hpf. (F) There is no S58 staining and weak zm4 staining in control RNA-injected yot embryos. (G) Misexpression of ff1a causes an increase of zm4 staining in yot mutants, but S58 staining remains absent. Scale bars are 20 μm.

Expression Data

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Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 286(2), Sheela, S.G., Lee, W.C., Lin, W.W., and Chung, B.C., Zebrafish ftz-f1a (nuclear receptor 5a2) functions in skeletal muscle organization, 377-390, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.