FIGURE

Fig. 5

ID

ZDB-FIG-070802-16

Publication

Schlueter et al., 2007 - Insulin-like growth factor receptor 1b is required for zebrafish primordial germ cell migration and survival

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Fig. 5

Ectopic PGCs undergo apoptosis. (A) Vasa immunostaining and (B) TUNEL analysis of ectopic PGCs in dorsal trunk of igf1rb-MO-injected embryo; (C; merged image) colocalization of Vasa-positive (arrows) and TUNEL-positive (arrowheads) cells confirms DNA fragmentation in ectopic PGCs. (D) Vasa-positive but (E) TUNEL-negative PGCs in genital ridge (circled) of igf1rb-MO-injected embryo; images merged in panel F. An absence of DNA fragmentation in genital ridge PGCs confirms survival of the subpopulation of PGCs that successfully migrate to the genital ridge. Anterior is to the left in all images; magnification, 200 x.

Expression Data

Gene:	ddx4
Fish:	WT + MO1-igf1rb + MO2-igf1rb
Knockdown Reagents:	MO1-igf1rb MO2-igf1rb
Anatomical Term:	primordial germ cell
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 305(1), Schlueter, P.J., Sang, X., Duan, C., and Wood, A.W., Insulin-like growth factor receptor 1b is required for zebrafish primordial germ cell migration and survival, 377-387, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.