FIGURE

Fig. 4

ID
ZDB-FIG-070628-3
Publication
Lin et al., 2007 - Depletion of Med10 enhances Wnt and suppresses Nodal signaling during zebrafish embryogenesis
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

Reduction of Med10 enhances Wnt signaling. (A–H) Reduction of Med10 results in expanded expression of Wnt target genes. MO1-Med10 (5 ng) was injected into embryos at the one-cell stage. Embryos were then fixed at either 30% epiboly (A–D) or 75% epiboly (E–H) and stained using in situ hybridization. Dorsal is right. Animal views are shown in (A–D); lateral views are shown in (E–H). eve1 expression was not affected by reduction in Med10 (A,B), serving as a control. Shown are spr2 expression in MO1-Med10 injected embryos (D,F) and control embryos (C,E); and cdx4 expression in MO1-Med10 injected embryos (H) and control embryos (G). (I–N) Reduction of Med10 leads to a graded expansion of lef1:gfp. Increasing amounts of MO1-Med10 were injected into embryos at the one-cell stage and in situ hybridization using a gfp riboprobe was performed on embryos at 30% (I–K) and 90% (L–N) epiboly. Animal pole views are shown in (I–K); lateral views are shown in (L–N). The region of gfp expression is increased in med10 morphants (J,K,M,N), as compared to control embryos (I,L). (O–Q) Reduction of Med10 enhances Wnt8-induced ectopic tail bud formation. wnt8/gfp mRNA (100 pg each) were injected into single cells at the animal pole of 128-cell stage embryos. Ectopic tail buds were induced in GFP-positive embryos (O), as confirmed by staining for tbx6 (P). Arrows indicate ectopic tail buds (O,P). (Q) Quantification of Wnt8-induced ectopic tail buds. Data presented in (Q) are the mean ± SD from at least three independent experiments. Total embryos scored were 663, 258, and 129 for wnt8/gfp, wnt8/gfp/MO1-Med10 (100 pg/100 pg/150 pg) and wnt8/gfp/med10 mRNA (100 pg each) injections, respectively.

Expression Data
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: 30%-epiboly to Shield

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 303(2), Lin, X., Rinaldo, L., Fazly, A.F., and Xu, X., Depletion of Med10 enhances Wnt and suppresses Nodal signaling during zebrafish embryogenesis, 536-548, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.