PUBLICATION

Depletion of Med10 enhances Wnt and suppresses Nodal signaling during zebrafish embryogenesis

Authors
Lin, X., Rinaldo, L., Fazly, A.F., and Xu, X.
ID
ZDB-PUB-070122-23
Date
2007
Source
Developmental Biology   303(2): 536-548 (Journal)
Registered Authors
Lin, Xueying, Xu, Xiaolei
Keywords
Transcriptional Mediator Complex (MED), Med10, Med12, Med13, Med15, Wnt, Nodal, Zebrafish
MeSH Terms
  • Transforming Growth Factor beta/metabolism*
  • Nodal Protein
  • Mutation
  • Sequence Homology, Amino Acid
  • Cloning, Molecular
  • Base Sequence
  • Signal Transduction
  • DNA Primers/genetics
  • Animals
  • Amino Acid Sequence
  • Wnt Proteins/metabolism*
  • Zebrafish Proteins/deficiency*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Molecular Sequence Data
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
(all 18)
PubMed
17208216 Full text @ Dev. Biol.
Abstract
The transcriptional Mediator (MED) is a multiprotein complex that transmits information from transcription factors to RNA polymerase II (PolII) to regulate transcription. At present, the role of distinct MED subunits in general transcription versus transcription stimulated by specific signaling pathways is unclear. By means of positional cloning, we reveal that the zebrafish mutant tennismatch is a hypomorphic allele of Med10, a conserved MED middle domain subunit. Using morpholino antisense oligonucleotides, we further demonstrate that reduction of Med10 levels led to an enhancement of the Wnt signaling pathway, while also suggesting a role for Med10 in mediating the Nodal signaling pathway. In contrast to the dual roles of Med10, reduction of Med12 and Med13 levels, two MED subunits in the regulatory domain, led to an enhancement of the Wnt signaling pathway but not the Nodal pathway, while reduction of Med15 levels, a MED subunit in the tail domain, suppressed the Nodal signaling pathway but not the Wnt signaling pathway. Thus, Med10 appears to be a unique MED subunit that differentially transduces information from distinct signaling pathways during zebrafish embryogenesis.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m686
    Unknown
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    med10MO1-med10MRPHLNO
    med10MO2-med10MRPHLNO
    med12MO4-med12MRPHLNO
    med13aMO1-med13aMRPHLNO
    med13aMO2-med13aMRPHLNO
    med15MO1-med15MRPHLNO
    med15MO2-med15MRPHLNO
    1 - 7 of 7
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    Fish
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    No data available
    Mapping