Fig. 2

Assessment of Heat Shock Induction and Levels of Hsp90 Inhibition (A) Western blots to assess Hsp70 induction following overnight treatment of WT embryos initiated at 50% epiboly with increasing concentrations of radicicol or 17AAG. As positive control, Hsp70 was induced by incubating embryos at 37 °C for 30–60 min before harvesting. (B) Western blots showing graded reduction in RAF1, an Hsp90 client-protein, in response to inhibition by radicicol and 17AAG. (C) Western blots to assess Hsp90, Hsp70, and RAF1 levels in embryos injected with 6 ng of universal morpholino control (STD MO) or Hsp90 a+b morpholinos at the indicated individual amounts. α-tubulin was used as loading control. *, Nonspecific band showing equal loading