FIGURE

Fig. 3

ID
ZDB-FIG-070619-32
Publication
Qian et al., 2007 - Distinct Functions for Different scl Isoforms in Zebrafish Primitive and Definitive Hematopoiesis
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Fig. 3

scl-α and -β Are Functionally Redundant in the Initiation of Primitive Hematopoiesis (A–L) WISH of gata1 (A–D), βe1-globin (E–H), and pu.1 (I–L). Similar expression of gata1, βe1-globin, and pu.1 were detected in 20-hpf control embryos (A, E, and I), scl-α morphants (B, F, and J), and scl-β morphants (C, G, and K). In contrast, expression of gata1, βe1-globin, and pu.1 were significantly reduced or absent in scl-α/β co-injected morphants (D, H, and L). All embryos are in lateral view with anterior to the left. (M–T) Immunohistochemistry staining of endogenous Scl proteins in 20-hpf control embryos (M and Q) and scl-α (N and R), scl-β (O and S), and scl-α/β co-injected (P and T) morphants using Ab-Scl-N (M–P) and Ab-Scl-C (Q–T). In scl-α morphants, the Ab-Scl-N (N), but not Ab-Scl-C (R), staining in the ICM was absent, showing that scl-α MO specifically blocks the translation of scl-α. In the scl-β morphants, the Ab-Scl-C staining was selectively abolished in the APLM, where only scl-β is transcribed ([S] asterisk, inset), demonstrating that scl-β MO specifically blocks the protein expression of scl-β. In the scl-α/β co-injected morphants, neither Ab-Scl-N nor Ab-Scl-C staining was detected (P and T). Insets in (Q–T) are dorsal views of the magnified APLM marked by asterisks. Embryos are in lateral view with anterior to the left.

Expression Data
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 20-25 somites

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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