ZFIN ID: ZDB-FIG-070413-17
Hsiao et al., 2007 - A Positive Regulatory Loop between foxi3a and foxi3b Is Essential for Specification and Differentiation of Zebrafish Epidermal Ionocytes. PLoS One   2(1):e302 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5

Fig. S5 Global down-regulation of epidermal ionocyte markers in foxi3a morphants. (A-F) Comparison of the epidermal ionocyte marker expression between wild-type (left panel) and foxi3a morphants (right panel). The expression of markers is completely abolished in the epidermal ionocyte lineage in foxi3a morphants. Note that the pronephric duct expressions in ndrg1, kcnj1, atp1a1a.2 and trpm7 were largely undisturbed, which shows that the foxi3a morphant phenotype specifically targets epidermal ionocytes. Na+,K+-ATPase-rich cell (NaRC) markers were ndrg1 (A), kcnj1 (B), and atp1a1a.2 (C). H+-ATPase-rich cell (HRC) markers were arg2 (D), trpm7 (E), and atp6voC (F). All embryos were scored at 24 hours post-fertilization (hpf).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
arg2 AB standard conditions Prim-5 epidermis ISH
AB + MO4-foxi3a standard conditions Prim-5 Not Detected epidermis ISH
atp1a1a.2 AB standard conditions Prim-5 epidermis ISH
Prim-5 pronephric duct ISH
AB + MO4-foxi3a standard conditions Prim-5 Not Detected epidermis ISH
Prim-5 pronephric duct ISH
atp6v0ca AB standard conditions Prim-5 epidermis ISH
AB + MO4-foxi3a standard conditions Prim-5 Not Detected epidermis ISH
kcnj1a.1 AB standard conditions Prim-5 epidermis ISH
Prim-5 pronephric duct ISH
AB + MO4-foxi3a standard conditions Prim-5 Not Detected epidermis ISH
Prim-5 pronephric duct ISH
ndrg1a AB standard conditions Prim-5 epidermis ISH
Prim-5 pronephric duct ISH
AB + MO4-foxi3a standard conditions Prim-5 Not Detected epidermis ISH
Prim-5 pronephric duct ISH
trpm7 AB standard conditions Prim-5 epidermis ISH
Prim-5 pronephric duct ISH
AB + MO4-foxi3a standard conditions Prim-5 Not Detected epidermis ISH
Prim-5 pronephric duct ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One