FIGURE

Fig. 7

ID

ZDB-FIG-070323-3

Publication

Pfeffer et al., 1998 - Characterization of three novel members of the zebrafish Pax2/5/8 family: dependency of Pax5 and Pax8 expression on the Pax2.1 (noi) function

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Fig. 7

Pax2.2 expression is independent of the noi (Pax2.1) function. Transcripts of the Pax2.2 gene were analysed by whole-mount in situ hybridization in wild-type (A,C) and noi^tu29a mutant (B,D) embryos at 7 somites and 20 hours postfertilization. The 7-somite embryos (A,B; lateral view) were genotyped by double staining with Pax2.2 (blue) and eng3 (red) RNA probes. Homozygous noi mutant embryos were identified by the absence of engrailed (eng) expression at the midbrain-hindbrain boundary (mhb) (Brand et al., 1996). At 20 hours, the mhb tissue is entirely lost in noi mutant embryos (dorsal view).

Expression Data

Genes:	en2b pax2b
Fish:	WT pax2a^tu29a/tu29a
Anatomical Terms:	midbrain hindbrain boundary midbrain hindbrain boundary neural keel optic stalk optic vesicle
Stage Range:	5-9 somites to 20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pax2a^tu29a/tu29a
Observed In:	brain midbrain hindbrain boundary midbrain-hindbrain boundary initiation
Stage Range:	5-9 somites to 20-25 somites

Phenotype Detail

Acknowledgments

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