FIGURE

Fig. 2

ID
ZDB-FIG-070228-2
Publication
Feng et al., 2006 - Hedgehog acts directly on the zebrafish dermomyotome to promote myogenic differentiation
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2

Hedgehog signaling negatively regulates the number of Pax7+ myogenic precursors. Embryos were labeled at the prim5 (24 h) stage for Pax7 (green) and myosin (MF20, red). (A) In wild-type embryos, the surface of each somite has two types of Pax7+ myogenic precursors, moderately labeled cells that are part of the somite, and more brightly labeled, dorso-ventrally elongated neural crest cell nuclei. (B, C) smu(smo)-/- and CyA-treated wild-type embryos have more Pax7+ myogenic precursors than wild-type control embryos, particularly in the anterior part of each somite (arrows). (D, E) In syu(shh)-/- and yot(gli2)-/- embryos, the number of Pax7+ nuclei is intermediate between untreated wild-type and smu(smo)-/- or CyA-treated wild-type embryos. (F) shh mRNA-injected wild-type embryos have virtually no Pax7+ nuclei. (G) A single optical slice of a CyA-treated wild-type embryo through the middle part of somites, demonstrating the gaps between myotomes that are filled with Pax7+ cells. Many of the Pax7+ cells are within the anterior of each somite (dotted outline). (H) Quantitation of the number of Pax7+ myogenic precursors per somite in each genotype or treatment (mean of 5 embryos per genotype or treatment, somites 16–18 were counted; error bar = standard deviation). Panels A–F are projections of Z-stack confocal sections (see Materials and methods), panel G is a thin optical slice, all panels are anterior to the left, dorsal up; somite 17 is shown in each panel. Scale bar = 50 μm.

Expression Data
Gene:
Fish:
Condition:
Anatomical Terms:
Stage: Prim-5

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Prim-5

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 300(2), Feng, X., Adiarte, E.G., and Devoto, S.H., Hedgehog acts directly on the zebrafish dermomyotome to promote myogenic differentiation, 736-746, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.