Fig. 7
- ID
- ZDB-FIG-061206-12
- Publication
- Lee et al., 2006 - Canonical Wnt signaling through Lef1 is required for hypothalamic neurogenesis
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Wnt8b and Lef1 act through the canonical Wnt pathway in the posterior hypothalamus. White circles outline posterior hypothalamus in whole-mount views, and black ovals outline posterior hypothalamus in cross-sections. (A,B) At 30 hpf, wnt8b (blue) and lef1 (red) show overlapping and adjacent expression in the posterior hypothalamus. (C-F) In wnt8b morphants, sox3, zash1a, dlx2 and isl1 expression are specifically reduced in the posterior hypothalamus at 30 hpf. (G-I) A transgenic reporter for ß-catenin-mediated transcription (TOPdGFP) shows mRNA expression in the posterior hypothalamus (red) at 24 hpf. In the posterior hypothalamus, wnt8b expression (blue-H) partially overlaps with gfp, whereas lef1 (blue-I) almost completely overlaps with gfp. (J-O) In TOPdGFP embryos, GFP protein (brown) is expressed in the posterior hypothalamus. At 24 hpf (J-L) and 30 hpf (M-O), this expression is absent in wnt8b and lef1 morphants. |
Genes: | (all 6) |
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Fish: | |
Knockdown Reagent: | |
Anatomical Term: | |
Stage Range: | Prim-5 to Prim-15 |
Fish: | |
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Knockdown Reagent: | |
Observed In: | |
Stage: | Prim-15 |