FIGURE

Fig. 6

ID

ZDB-FIG-060828-12

Publication

Raeker et al., 2006 - Obscurin is required for the lateral alignment of striated myofibrils in zebrafish

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Fig. 6

Obscurin depletion disrupts somite architecture. A: Embryos injected with MO2 (6 ng; A.2) or a control morpholino CMO2 (6 ng; A.1) were fixed and immunostained for α-actinin at 72 hpf. Note that in the morphant embryos, there are no detectable horizontal myoseptae (>) and only rudimentary transverse myoseptae (∧) compared to control embryos. Elongated, disarrayed myofibrils often extend beyond the length of a normal somite. B-D: Electron micrographs of transverse myoseptae from control [6 ng CMO2 (B)] and obscurin morphant [3 ng (C) and 6 ng (D) of MO2] embryos. Morphant embryos displayed rudimentary transverse myoseptae (C:*, C,D: <) at the ends of the skeletal myocytes compared to the well-organized transverse myoseptae of control embryos (B: *).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	EKW + MO2-obscnb
Knockdown Reagent:	MO2-obscnb
Observed In:	horizontal myoseptum skeletal muscle cell myofibril vertical myoseptum
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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