CRISPR

CRISPR1-adcy3a

ID

ZDB-CRISPR-240126-3

Name

CRISPR1-adcy3a

Previous Names

None

Target

adcy3a (1)

Sequence

5' - CAACAAGATGGAGGCCGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf3713	adcy3a
zf3714	adcy3a

Expression

Gene expression in Wild Types + CRISPR1-adcy3a

No data available

Phenotype

Phenotype resulting from CRISPR1-adcy3a

No data available

Phenotype of all Fish created by or utilizing CRISPR1-adcy3a

Phenotype	Fish	Conditions	Figures
integument mitfa expression decreased amount, abnormal	adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715}	standard conditions	Figure 3 from Zhang et al., 2022
integument tyr expression decreased amount, abnormal	adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715}	standard conditions	Figure 3 from Zhang et al., 2022
integument decreased pigmentation, exacerbated	adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715}	constant dark	Figure 2 from Zhang et al., 2022
integument tyrp1b expression decreased amount, abnormal	adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715}	standard conditions	Figure 3 from Zhang et al., 2022
integument melanosome dispersed, abnormal	adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715}	chemical treatment by environment: dibutyryl-cAMP	Figure 4 from Zhang et al., 2022
integument melanosome dispersed, abnormal	adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715}	chemical treatment by environment: 3,5-Dibromo-N'-((2,5-dimethyl-1-(3-nitrophenyl)-1H-pyrrol-3-yl)methylene)-4-hydroxybenzohydrazide	Figure 5 from Zhang et al., 2022
caudal fin melanosome aggregated, abnormal	adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715}	chemical treatment by environment: alpha-melanocyte stimulating hormone	Figure 4 from Zhang et al., 2022
integument melanosome aggregated, abnormal	adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715}	control	Figure 4 , Figure 5 from Zhang et al., 2022
melanophore stripe absence of anatomical entity, abnormal	adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715}	standard conditions	Figure 3 from Zhang et al., 2022
anal fin melanosome aggregated, abnormal	adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715}	standard conditions	Figure 3 from Zhang et al., 2022
dorsal fin melanosome aggregated, abnormal	adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715}	standard conditions	Figure 3 from Zhang et al., 2022
integument melanocyte dispersed, abnormal	adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715}	standard conditions	Figure 3 from Zhang et al., 2022
integument melanosome localization process quality, exacerbated	adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715}	constant dark	Figure 2 from Zhang et al., 2022
integument melanocyte irregular spatial pattern, exacerbated	adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715}	constant dark	Figure 2 from Zhang et al., 2022
integument melanosome dispersed, abnormal	adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715}	chemical treatment by environment: ciliobrevin D	Figure 5 from Zhang et al., 2022
melanocyte melanosome punctate, abnormal	adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715}	standard conditions	Figure 3 from Zhang et al., 2022
caudal fin melanosome aggregated, abnormal	adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715}	control	Figure 3 , Figure 4 from Zhang et al., 2022
caudal fin melanosome dispersed, abnormal	adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715}	chemical treatment by environment: dibutyryl-cAMP	Figure 4 from Zhang et al., 2022
integument dct expression decreased amount, abnormal	adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715}	standard conditions	Figure 3 from Zhang et al., 2022
integument melanosome dispersed, abnormal	adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715}	chemical treatment by environment: alpha-melanocyte stimulating hormone	Figure 4 from Zhang et al., 2022
integument melanosome dispersed, abnormal	adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715} + MO1-dync1h1	control	Figure 5 from Zhang et al., 2022

Citations