FIGURE SUMMARY
Title

epg5 knockout leads to the impairment of reproductive success and courtship behaviour in a zebrafish model of autophagy-related diseases

Authors
Fontana, C.M., Locatello, L., Sabatelli, P., Facchinello, N., Lidron, E., Maradonna, F., Carnevali, O., Rasotto, M.B., Dalla Valle, L.
Source
Full text @ Biomed J
Fig. 1

Fig. 1. Schematic representation of the tank used for behavioural mating trials. Image created with BioRender.com.

Fig. 2

Fig. 2. Breeding performance of epg5+/+ and epg5−/− males. (A) Number of successful breeding events in 4 reproductive rounds (n = 12 epg5+/+ and 13 epg5−/−) and (B) number of eggs spawned by WT females in successful breeding events (n = 12 epg5+/+ and 6 epg5−/−). Boxplots (first to third quartile) with median line and whiskers (1.5 interquartile range). ∗∗p < 0.01.
Fig. 3

Fig. 3. Sperm viability in epg5+/+ (n = 11) and epg5−/− (n = 12) males. Viability is calculated as the percentage of alive sperm. Boxplots (first to third quartile) with median line and whiskers (1.5 interquartile range). ∗p < 0.05.

PHENOTYPE:
Fish:
Observed In:
Stage: Adult
Fig. 4

Fig. 4. Histology of the testes of epg5+/+ (A) and epg5−/− (B, C, D) males. Males (A), (B) and (C) showed 100% of breeding success in previous reproductive trials, whereas male (D) did not reproduced. Scale bar 50 μm sg = spermatogonia; sc = spermatocytes; st = spermatids; sz = spermatozoa.

PHENOTYPE:
Fish:
Observed In:
Stage: Adult
Fig. 5

Fig. 5. Transmission electron microscope on sperm of epg5+/+ and epg5−/− adult zebrafish. Representative transversal sections showing no obvious differences in morphology of nuclei (n) and mitochondria (m) of (A) epg5+/+ and (B) epg5−/− sperm. Scale bar, 200 nm.

PHENOTYPE:
Fish:
Observed In:
Stage: Adult
Fig. 6

Fig. 6. Male and female reproductive behaviours. epg5+/+ (n = 8) and epg5−/− (n = 7) males were coupled to WT females. (A) Time spent by the couple in shallow water area; (B) number of male encircles; (C) number of male quivers; (D) time spent by the female approaching and chasing the male. Boxplots (first to third quartile) with median line and whiskers (1.5 interquartile range). ∗p < 0.05. Fish icons created with BioRender.com

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biomed J
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